Triple fixation of Bacillus subtilis dormant spores

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Permeability of dormant spores of Bacillus subtilis to gramicidin S.

Gramicidin S, dissolved in ethanol, penetrated into the inside of the dormant spores of Bacillus subtilis, had a partial inhibitory effect on L-alanine-initiated germination and completely inhibited their outgrowth and vegetative growth. The activity of particulate NADH oxidase of the antibiotic-treated dormant spores was also influenced significantly. Abnormal morphological changes were observ...

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Water and Small-Molecule Permeation of Dormant Bacillus subtilis Spores.

UNLABELLED We use a suspended microchannel resonator to characterize the water and small-molecule permeability of Bacillus subtilis spores based on spores' buoyant mass in different solutions. Consistent with previous results, we found that the spore coat is not a significant barrier to small molecules, and the extent to which small molecules may enter the spore is size dependent. We have devel...

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Levels of germination proteins in dormant and superdormant spores of Bacillus subtilis.

Bacillus subtilis spores that germinated poorly with saturating levels of nutrient germinants, termed superdormant spores, were separated from the great majority of dormant spore populations that germinated more rapidly. These purified superdormant spores (1.5 to 3% of spore populations) germinated extremely poorly with the germinants used to isolate them but better with germinants targeting ge...

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Levels of Germination Proteins in Bacillus subtilis Dormant, Superdormant, and Germinating Spores

Bacterial endospores exhibit extreme resistance to most conditions that rapidly kill other life forms, remaining viable in this dormant state for centuries or longer. While the majority of Bacillus subtilis dormant spores germinate rapidly in response to nutrient germinants, a small subpopulation termed superdormant spores are resistant to germination, potentially evading antibiotic and/or deco...

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Quasi-elastic light scattering studies on dormant and germinating Bacillus subtilis spores.

Spores of Bacillus subtilis in suspension, both dormant and germinating, have been examined by light-scattering methods, both integrated intensity and correlation versions. When intensity of scatter at constant volume was plotted against angle, curves possessing a maximum at about 20 degrees were regularly obtained but without any noticeable features at higher angles. This indicated polydispers...

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1983

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.156.1.409-413.1983